- Entry level
- No Education
- Salary to negotiate
The French National Research Institute for Agriculture, Food, and the Environment (INRAE) is a public research establishment.It is a community of 12,000 people with more than 200 research units and 42 experimental units located throughout France.The institute is among the world leaders in agricultural and food sciences, in plant and animal sciences, and is 11th in the world in ecology and environment. INRAE’s main goal is to be a key player in the transitions necessary to address major global challenges. In the face of the increase in population, climate change, scarcity of resources and decline in biodiversity, the institute develops solutions for multiperformance agriculture, high quality food and sustainable management of resources and ecosystems.
Work environment, missions and activities
Autophagy (literally meaning, "self-eating") is an evolutionarily conserved process in eukaryotes that encompasses different types of mechanisms by which cells deliver cytoplasmic constituents to the lysosome for degradation and whose defects have been associated with several human pathologies.
Interestingly, two different autophagic pathways, (i) the Chaperone-Mediated Autophagy (CMA) and (ii) the endosomal microautophagy (eMI), both rely on the use of the same cytosolic chaperone HSPA8 (also known as HSC70) for targeting substrates to the lysosome. Although our knowledge of the mechanisms specifically involved within these two distinct autophagic routes has greatly improved in recent years, little is currently known about their molecular and physiological interrelationships. In particular, the mechanisms underlying the transport of substrates by HSPA8 to either degradation pathway remain to be explained. In this context, we propose to use the medaka model fish species (Oryzias latipes) to decipher and improve our knowledge regarding to the existing interplays between CMA and eMI. Due to their short generation time, their relatively small genome (~800 mega base pairs, half the size of the genome of another popular model fish, the zebrafish), and the possibility to produce transgenic animals, medaka emerged these last years as an excellent model system in various fields of biology.
The PhD project will be conducted under the co-supervision of Iban SEILIEZ (Research Director) and Amaury HERPIN (researcher). Research carried out by Iban SEILIEZ aims, through in vivo and in vitro studies, to improve our understanding of the nutritional control of autophagy and its role in the regulation of metabolism in teleost fish. I.S. has produced and/or participated to the production of original results published in 63 articles in international journals. He participated to or managed several collaborative national (Conseil regional Aquitaine, ANR, FEAMP) and international (NORC from the University of Alabama) research projects. Researches performed by Amaury HERPIN are focused on the use of medaka as a model organism for studying gonadal development via functional genomics approaches. He developed know-how on gene editing in this model and has been involved in the production of original results published in more than 40 articles. Overall, this PhD project will benefit of the synergy between these two partners with highly complementary expertise and skills in autophagy and fish genomics.
Training and skills
PhD Solid bases in molecular biology are required. Good communication skills in oral and written English are essential.
INRAE's life quality
By joining our teams, you benefit from:
- 30 days of annual leave + 15 days "Reduction of Working Time" (for a full time);
-parenting support: CESU childcare, leisure services;
- skills development systems:training,career advise;
-social support: advice and listening, social assistance and loans;
-holiday and leisure services: holiday vouchers, accommodation at preferential rates;
-sports and cultural activities;
- collective catering.
About the company