- Entry level
- No Education
- Salary to negotiate
- Spring House
Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for an Associate Scientist, Lentivirus & CAR-T Production located in Spring House, PA.
At the Janssen Pharmaceutical Companies of Johnson & Johnson, what matters most is helping people live full and healthy lives. We focus on treating, curing and preventing some of the most devastating and complex diseases of our time. And we pursue the most promising science, wherever it might be found.
Janssen Research & Development, LLC discovers and develops innovative medical solutions to address important unmet medical needs in oncology, immunology, neuroscience, infectious diseases and vaccines, and cardiovascular and metabolic diseases. Please visit http://www.janssenrnd.com/ for more information.
We are Janssen. Our mission drives us. Our patients inspire us. We collaborate with the world for the health of everyone in it.
Thriving on a diverse company culture, celebrating the uniqueness of our employees and committed to inclusion. Proud to be an equal opportunity employer.
Biotherapeutics works on projects from five Therapeutic Areas (TA) and enables discovery of new therapeutic concepts in collaboration with TA discovery counterparts. Supporting the CAR-T Platform group. This position will be accountable for early discovery efforts to engineer CAR-T cells for initial functional characterization in conjunction with our team and Oncology Therapeutic Area stakeholders. Key responsibilities include, but are not limited to: daily maintenance of cell cultures to enable the production of lentiviral particles and/or CAR-T cells; working knowledge of viral-based production technologies and quality control (QC) techniques; ability to transduce primary human T-cells with both lenti- and adeno-associated viral particles; utilize flow cytometric techniques to determine transduction efficiency and to perform related QC measurements of engineered cells; provide functional and intellectual support to the CAR-T cell group within Biotherapeutics.
Strong experience in research-scale lentivirus vector/particle generation
Working knowledge of assays that measure infectious and physical viral titer
Expertise in primary T cell gene transfer via lentiviral transduction
Isolation of primary human immune cells, including T-lymphocytes, from human blood and subsequent aseptic cell culture
Phenotypically characterize genetically modified primary T-lymphocytes using flow cytometric instrumentation
Viral vector-based or electroporation-based modification of tumor cell lines and primary human T-cells
Maintain an electronic lab notebook and contribute to support documentation in line with company standards
Support the CAR-T R&D team in JBIO to enable timely development and delivery of novel molecular entities to therapeutic area stakeholders
Self-motivated and eager to take on temporary responsibilities outside of initial job description
B.S with 1-3 years of T cell biology experience in academic or industrial background
Minimum of 2 years’ experience with lentiviral-based gene delivery (primary T-cell preferred)
Ability to work independently with advanced flow cytometry equipment
Expertise in mammalian cell culture, particularly with primary human T-cells, is essential
Outstanding collaborative and communication skills
Foster a working environment that encourages the company's commitment to equal employment opportunity and the value of a diverse work force
United States-Pennsylvania-Spring House-1400�McKean�Road
Janssen Research & Development, LLC. (6084)
About the company
If you’re thinking about taking your career to a place where you can be seen for the talent you bring to your work, where you’ll have a chance to be the driver of your own career, and where others share your passion for caring and success, then we invite you to explore Johnson & Johnson. BE VITAL in your career and make a unique mark. Join the approximately 129,000 men and women who work within our companies and touch the lives of more than a billion people around the world every day.